IL17 producing Th17 cells playing a complex role in tumor immunity. Induced by TGF-β, IL6, and IL1β, and controlled by the transcription factor RORγt, Th17 cells secrete cytokines which can have either a beneficial or detrimental effect depending on multiple factors.
To help you study this subject efficiently, we have prepared a complete protocol to isolate mouse CD4+ cells and accurately monitor Th17 differentiation.
Featured in this application note:
- CD4+ T-cell isolation protocol,
- Th17 differentiation as followed by FACS, ELISA, and HTRF,
- Valuable data showing the HTRF method’s benefits.
