Guidelines from PBMC isolation to cytokine assay optimization

Perform and optimize cytokine assays on PBMC

This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF. Take a good look at the protocols and examples it features!


  • PBMC isolation protocol
  • HTRF basics and cytokine assays
  • Optimization and examples