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App Note

Advantages of Using our Automatable and No-Wash HTRF® and AlphaLISA® Kits for CHO HCP Detection

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Detecting and Quantifying Them With Automatable Homogeneous Immunoassays

During biotherapeutic manufacturing and production, the host cells - a great majority of them being Chinese Hamster Ovary (CHO) cells – produce protein impurities that are called Host Cell Proteins (HCPs). Even if more than 99% of them are removed from the final product, the residual CHO HCPs can induce immunogenicity in individuals or reduce the potency, stability, or effectiveness of a drug. Therefore, to meet regulatory organizations’ guidelines (such as FDA or EMA) on CHO HCP levels, biopharmaceutical companies spend significant amounts of money on tools and strategies for their detection.

Illustrated with robust results, this Application Note explains the many ways in which HTRF® and AlphaLISA® Kits can improve the workflow for CHO HCP detection:

  1. Precision and wide antibody coverage

  2. Compatibility with most commonly used buffers

  3. No cross-reactivity between CHO HCP detection and drug substance

  4. Good dilutional linearity and antigen spike recovery

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