App Note

HTRF analysis and imaging of phospho-S65-ubiquitin in cellular models of mitophagy

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Phospho-S65-Ubiquitin-1 Application Note

Studying mitophagy activation in relation to ubiquitin phosphorylation with HTRF

Recent research studies have established that defective mitophagy constitutes one of the hallmarks of neurodegenerative disease onset, such as Alzheimer’s and Parkinson’s disease. Ubiquitin phosphorylation at serine 65 is proven to be a specific marker of ubiquitin-dependent PINK1/Parkin-mediated mitophagy induction, with PINK1/Parkin linked to Parkinson’s disease.

In this application note we will show you how to investigate mitophagy activation in relation to phospho-ubiquitin (Ser 65) signaling using HTRF, a fast and sensitive solution compared to standard assays and imaging.


  • Background on mitophagy, ubiquitin, and PINK1/Parkin Pathway involvement in neurodegenerative diseases
  • Results showing the ability to test compounds with HTRF that may modulate the PINK1/Parkin pathway
  • Materials and methods to perform these experiments

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