Harness avidity without compromising on pharmacology
Characterize compounds in extra-low affinity interactions
This Note groups protocols and data showing how a Biotin-Streptavidin system can be used to increase the apparent affinity of a protein-protein interaction and make it easy to characterize.
No need to worry about the pharmacology of compounds tested this way: you will also see experiments demonstrating that this avidity-based approach does not impair compound characterization.
Features
Apparent affinity of weak interactions enhanced with the Biot-SA system
Evidence for pharmacological validation
Kd determination using the avidity approac
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