App Note

AAV Capsid Detection and Characterization with New AlphaLISA Assay Kits

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Application Note

Sensitive, Reliable, Quantitative, and No-Wash Assay Platform for AAV Capsid Detection

AAVs are widely used as viral vectors in human gene therapy implementing recombinant DNA technology. Accurate measurement of AAV concentration in complex biological matrices is essential for safe clinical application and effective manufacturing of AAV gene therapies.

Revvity has developed and manufactured a line of immunoassays that detect and quantify AAV capsid with AlphaLISA® technology. These assays can detect AAV1, AAV2, AAV3B, AAV5, AAV6, AAV8, and AAV9 serotypes, and measure AAV particles present in cell culture media, lysis buffer, and cell lysate.

Get Your Application Note To:

  • Learn more about AAV capsid detection kits
  • Access data on detection limit, assay reproducibility, variability, specificity, and recovery from multiple analyte matrices through explained diagrams
  • See details on experimental conditions

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